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《化学科学与工程前沿(英文)》 2021年 第15卷 第6期 页码 1572-1582 doi: 10.1007/s11705-021-2112-4
关键词: reduced graphene oxide gold nanoparticles electrochemical biosensor cholesterol oxidase cholesterol
Self-assembly of metal-cholesterol oxidase hybrid nanostructures and application in bioconversion of
Yu Xin, Qiuyue Gao, Yu Gu, Mengyao Hao, Guangming Fan, Liang Zhang
《化学科学与工程前沿(英文)》 2021年 第15卷 第3期 页码 615-629 doi: 10.1007/s11705-020-1989-7
关键词: cholesterol oxidase metal-enzyme hybridization nanostructures sterol derivatives bioconversion
Insight into the role of cholesterol in modulation of morphology and mechanical properties of CHO-K1
Lei Zhang, Lisha Zhao, Ping-Kai Ouyang, Pu Chen
《化学科学与工程前沿(英文)》 2019年 第13卷 第1期 页码 98-107 doi: 10.1007/s11705-018-1775-y
Cholesterol plays a significant role in the organization of lipids and modulation of membrane dynamics in mammalian cells. However, the effect of cholesterol depletion on the eukaryotic cell membranes seems controversial. In this study, the effects of cholesterol on the topography and mechanical behaviors of CHO-K1 cells with manipulated membrane cholesterol contents were investigated by atomic force microscopy (AFM) technique. Here, we found that the depletion of cholesterol in cell membranes could increase the membrane stiffness, reduce the cell height as well as promote cell retraction and detachment from the surface, whereas the cholesterol restoration could reverse the effect of cholesterol depletion on the membrane stiffness. Increased methyl-β-cyclodextrin levels and incubation time could significantly increase Young’s modulus and degree of stiffing on cell membrane and cytoskeleton. This research demonstratede importance of cholesterol in regulating the dynamics of cytoskeleton-mediated processes. AFM technique offers excellent advantages in the dynamic monitoring of the change in membranes mechanical properties and behaviors during the imaging process. This promising technology can be utilized in studying the membrane properties and elucidating the underlying mechanism of distinct cells in the near-native environment.
关键词: cholesterol methyl-β-cyclodextrin atomic force microscopy Young’s modulus CHO-K1 cell
标题 作者 时间 类型 操作
DNA-reduced graphene oxide nanocomposites based electrochemical biosensor for highly sensitive detection of cholesterol
期刊论文
Self-assembly of metal-cholesterol oxidase hybrid nanostructures and application in bioconversion of
Yu Xin, Qiuyue Gao, Yu Gu, Mengyao Hao, Guangming Fan, Liang Zhang
期刊论文